Mycoplasma Removal Reagent
Mycoplasma Removal Reagents (MRRs) are specialized solutions used in cell culture and laboratory settings to eliminate Mycoplasma contamination from cell cultures. These reagents are formulated to target and eradicate Mycoplasma species—small, wall-less bacteria that are notorious for contaminating cell cultures—while minimizing harm to the host cells. Proper use of MRRs ensures the integrity of experimental results, maintains cell line quality, and prevents the spread of contamination between cultures.
Content:
- Composition and Mechanism:
- Active Ingredients:
- MRRs typically contain a blend of antibiotics, antimicrobial peptides, or chemical agents specifically effective against Mycoplasma. Common compounds may include quinolones, macrolides, tetracyclines, or proprietary blends that target unique aspects of Mycoplasma cell membranes or metabolic pathways.
- Mechanism of Action:
- Due to the lack of a cell wall in Mycoplasma, typical antibiotics like penicillin are ineffective. MRRs instead use agents that disrupt membrane integrity, inhibit protein synthesis, or interfere with DNA replication in Mycoplasma cells.
- Active Ingredients:
- Application Protocol:
- Preparation:
- Before application, verify the cell culture’s viability and assess the degree of Mycoplasma contamination via testing methods like PCR, ELISA, or fluorescent staining.
- Preparation:
- Treatment Process:
- Add the recommended concentration of MRR directly to the contaminated cell culture medium. In some protocols, cells may need to be incubated with the reagent for a specified duration, often spanning several days, with periodic medium changes to ensure effective removal.
- Post-Treatment:
- After the treatment period, perform a thorough wash or medium replacement to remove residual reagent from the culture.
- Treatment Process:
Mycoplasma Removal Reagents are vital tools for maintaining uncontaminated cell cultures. By specifically targeting Mycoplasma organisms without significantly affecting host cells, these reagents preserve the integrity of experimental data and support reliable biological research. Proper application, adherence to protocols, and continuous monitoring are key to successfully eliminating Mycoplasma contamination and ensuring long-term cell culture health.
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